xviii, 740 pages : 29 cm

A unique, adaptable textbook for upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. Allows instructors to easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications

Includes bibliographical references and index

Machine generated contents note: Emergence of Molecular Biotechnology -- Recombinant DNA Technology -- Commercialization of Molecular Biotechnology -- Concerns and Consequences -- Summary -- References -- Review Questions -- Molecular Cloning -- Preparation of DNA for Cloning -- Insertion of Target DNA into a Plasmid Vector -- Transformation and Selection of Cloned DNA in a Bacterial Host -- Cloning Eukaryotic Genes -- Recombinational Cloning -- Genomic Libraries -- Genome Engineering using CRISPR Technology -- Polymerase Chain Reaction -- Amplification of DNA by PCR -- Cloning PCR Products -- Quantitative PCR -- Chemical Synthesis of DNA -- Synthesis of Oligonucleotides -- Assembling Oligonucleotides into Genes -- Gene Synthesis by PCR -- DNA Sequencing Technologies -- Dideoxynucleotide Procedure -- Pyrosequencing -- Sequencing using Reversible Chain Terminators -- Sequencing by Single Molecule Synthesis -- Sequencing Whole Genomes

Note continued: Preparation of Genomic DNA Sequencing Libraries -- High-Throughput Next-Generation Sequencing Strategies -- Genome Sequence Assembly -- Sequencing Metagenomes -- Genomics -- Transcriptomics -- Proteomics -- Metabolomics -- Summary -- References -- Review Questions -- Protein Production in Prokaryotic Hosts -- Regulation of Transcription -- Increasing Translation Efficiency -- Increasing Protein Stability -- Increasing Protein Secretion -- Facilitating Protein Purification -- DNA Integration into the Host Chromosome -- Heterologous Protein Production in Eukaryotic Cells -- Posttranslational Modification of Eukaryotic Proteins -- General Features of Eukaryotic Expression Systems -- Yeast Expression Systems -- Baculovirus-Insect Cell Expression Systems -- Mammalian Cell Expression Systems -- Protein Engineering -- Directed Mutagenesis -- Random Mutagenesis -- Examples of Protein Engineering -- Summary -- References -- Review Questions

Note continued: Immunological Approaches to Detect Protein Biomarkers -- Antibodies -- Agglutination -- Enzyme-Linked Immunosorbent Assays -- Protein Arrays to Detect Polygenic Diseases -- Immunoassays for Protein Conformation-Specific Disorders -- DNA-Based Diagnostic Approaches -- Hybridization Probes -- PCR-Based Detection Methods -- DNA Microarrays -- Whole Genome Sequencing to Assess Genetic Disease Risk -- Detecting RNA Signatures of Disease -- Detection of Disease-Associated Changes in Gene Expression Using Microarrays -- Detection of RNA Signatures of Antibiotic Resistance in Bacteria -- Detection of miRNA Signatures of Cancers -- Biofluorescent and Bioluminescent Systems -- Fluorescent Proteins -- Luciferase -- Microbial Biosensors -- Summary -- References -- Review Questions -- Pharmaceuticals -- Human Interferons -- Human Growth Hormone -- Tumor Necrosis Factor Alpha -- Extending Protein Half-Life -- Enzymes -- DNase I -- Alginate Lyase

Note continued: Phenylalanine Ammonia Lyase -- alpha1-Antitrypsin -- Glycosidases -- Masking Nonhuman Epitopes -- Engineering Bacteriophages -- Targeting Mitochondria -- Lactic Acid Bacteria -- Interleukin-10 -- Leptin -- An HIV Inhibitor -- Insulin -- Recombinant Antibodies -- Hybrid Human-Mouse Monoclonal Antibodies -- Human Monoclonal Antibodies -- Antibody Fragments -- Combinatorial Libraries of Antibody Fragments -- A Combinatorial Library of Full-Length Antibodies -- Shuffling CDR Sequences -- Dual-Variable-Domain Antibodies -- Anticancer Antibodies -- Antibodies Against Various Diseases -- Antiobesity Antibodies -- Enhanced Antibody Half-Life -- Summary -- References -- Review Questions -- Targeting Specific mRNA and DNA Sequences -- Antisense RNA -- Aptamers -- Ribozymes and DNAzymes -- Interfering RNA -- Zinc Finger Nucleases -- CRISPR-Cas System -- Nanozymes -- Nanoparticles -- Viral Delivery Systems -- Nonviral Delivery Systems -- Direct Injection -- Lipids

Note continued: Bacteria -- Dendrimers -- Antibodies -- Aptamers -- Transposons -- Gene Therapy -- Prodrug Activation Therapy -- Promoterless Gene Targeting -- Summary -- References -- Review Questions -- Vaccination -- Current and Future Vaccines -- Subunit Vaccines -- Herpes Simplex Virus -- Cholera -- SARS -- Staphylococcus aureus -- Human Papillomavirus -- Foot-and-Mouth Virus -- Streptococcus -- Delivery -- Peptide Vaccines -- Malaria -- Genetic Immunization: DNA Vaccines -- Delivery -- Cancer -- Dental Caries -- Attenuated Vaccines -- Herpes Simplex Virus -- Cholera -- Salmonella Species -- Leishmania Species -- Vector Vaccines -- Vaccines Directed against Viruses -- Vaccines Directed against Bacteria -- Bacteria as Antigen Delivery Systems -- Monoclonal Antibody Passive Immunity -- Influenza Virus -- Summary -- References -- Review Questions -- Restriction Endonucleases -- Small Biological Molecules -- L-Ascorbic Acid -- Indigo -- Amino Acids -- Lycopene

Note continued: Antibiotics -- Biopolymers -- Microbial Degradation of Xenobiotics -- Genetic Engineering of Biodegradative Pathways -- Utilization of Starch and Sugars -- Commercial Production of Fructose and Alcohol -- Increasing Alcohol Production -- Improving Fructose Production -- Utilization of Cellulose and Hemicellulose -- Lignocellulosics -- Cellulase Genes -- Direct Conversion of Biomass to Ethanol -- Zymomonas mobilis -- Lipids from Cyanobacteria -- Hydrogen Production -- Summary -- References -- Review Questions -- Principles of Microbial Growth -- Batch Fermentation -- Fed-Batch Fermentation -- Continuous Fermentation -- Maximizing The Efficiency of The Fermentation Process -- High-Density Cell Cultures -- Increasing Plasmid Stability -- Quiescent E. Coli Cells -- Protein Secretion -- Reducing Acetate -- Bioreactors -- Typical Large-Scale Fermentation Systems -- Two-Stage Fermentation in Tandem Airlift Reactors

Note continued: Two-Stage Fermentation in a Single Stirred-Tank Reactor -- Batch versus Fed-Batch Fermentation -- Harvesting Microbial Cells -- Disrupting Microbial Cells -- Downstream Processing -- Protein Solubilization -- Utilizing an Immobilized Enzyme -- Magnetic Separation of Proteins -- Large-Scale Production of Plasmid DNA -- Summary -- References -- Review Questions -- Plant Transformation with the Ti Plasmid of A. Tumefaciens -- Ti Plasmid-Derived Vector Systems -- Microprojectile Bombardment -- Chloroplast Engineering -- Very High Level Protein Expression -- Use of Reporter Genes in Transformed Plant Cells -- Manipulation of Gene Expression in Plants -- Transient Gene Expression -- Plant Promoters -- Targeted Gene Editing -- Facilitating Protein Purification -- Protein Glycosylation -- Production of Marker-Free Transgenic Plants -- Removing Marker Genes from Nuclear DNA -- Removing Marker Genes from Chloroplast DNA -- Summary -- References

Note continued: Review Questions -- Insect Resistance -- B. thuringiensis Insecticidal Toxin -- Increasing Expression of the B. thuringiensis Protoxin -- Other Strategies for Protecting Plants against Insects -- Preventing the Development of B. thuringiensis-Resistant Insects -- Targeting Aphids -- Virus Resistance -- Viral Coat Protein-Mediated Protection -- Protection by Expression of Other Genes -- Herbicide Resistance -- Fungus and Bacterium Resistance -- Salt and Drought Stress -- Fruit Ripening and Flower Wilting -- Modification of Plant Nutritional Content -- Amino Acids -- Lipids -- Vitamins -- Iron -- Gluten -- Modification of Food Plant Taste and Appearance -- Preventing Discoloration -- Starch -- Plants as Bioreactors -- Antibodies -- Poly(3-hydroxybutyric Acid) -- Eddible Vaccines -- Plant Yield -- Altering Lignin Content -- Increasing Oxygen Content -- Summary -- References -- Review Questions -- Transgenic Mice: Methodology -- DNA Microinjection Method

Note continued: Retroviral Vector Method -- Engineered Embryonic Stem Cell Method -- Conditional Gene Inactivation with the Cre-IoxP Recombination System -- Genome Editing with the CRISPR-Cas System -- Gene Knockdown by RNA Interference -- Transgenic Mice: Applications -- Transgenic Disease Models: Alzheimer Disease -- Transgenic Mice as Test Systems -- Control of Transgene Expression -- Conditional Control of Cell Death -- Transgenic Livestock -- Cloning Livestock by Somatic Cell Nuclear Transfer -- Production of Pharmaceuticals -- Production of Donor Organs -- Disease Resistant Livestock -- Improving Milk Quality -- Improving Animal Production Traits -- Transgenic Poultry -- Transgenic Fish -- Summary -- References -- Review Questions -- Development of Guidelines for Recombinant DNA Research -- Deliberate Release of Genetically Modified Microorganisms -- Environmental Concerns -- Regulations -- Regulation of Genetically Modified Foods

Note continued: Food Ingredients Produced by Genetically Engineered Microorganisms -- Genetically Modified Crops -- Genetically Engineered Livestock -- Societal Concerns About Genetically Modified Foods -- Alteration of Nutritional Content of Food -- Potential for Introducing Toxins or Allergens into Food -- Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms -- Controversy About the Labeling of Genetically Modified Foods -- Impact of Genetically Engineered Crops on Biodiversity -- Who Benefits from Production of Genetically Modified Foods? -- Environmental Benefits of Genetically Modified Crops -- How do Views about Genetically Engineered Organisms Impact Trade? -- Regulation and Safety of Medical Products of Biotechnology -- New Biological Drugs -- Genetic and Genomic Testing -- Economic Issues -- Patenting Biotechnology -- Patenting -- Patenting in Different Countries -- Patenting Nucleic Acid Sequences

Note continued: Patenting Living Organisms -- Patenting and Fundamental Research -- Summary -- References -- Review Questions

plus-circle Add Review

comment

Reviews